• Express System: HEK293
  • Product Tag: C-Llama IgG2b Fc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PD-1, Llama Fc Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 45.2ng/ml determined by ELISA.
  • Amino Acid Range: Leu25-Gln167
Catalog #PD1-HM601
Price
Regular price $330.00
Regular price Sale price $330.00
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Size
Avantor VWR Fisher Scientific

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Human PD-1/PDCD1 Protein (PD1-HM601) Summary

Product Description

 Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with Llama IgG2b Fc tag at the C-terminus.It contains Leu25-Gln167.

Product Background

 Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.

Product Category

 Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human PD-1/PDCD1 Protein (PD1-HM601) Specifications/Details

Protein
 PD-1/PDCD1
Synonyms
 PDCD1; PD1; CD279; SLEB2; PD-1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu25-Gln167
Molecular Weight
 The protein has a predicted MW of 43.26 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human PD-1, Llama Fc Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 45.2ng/ml determined by ELISA.
Product Tag
 C-Llama IgG2b Fc

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human PD-1, Llama Fc Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 45.2ng/ml determined by ELISA. Immobilized Human PD-1, Llama Fc Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 45.2ng/ml determined by ELISA.
Human PD-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human PD-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Biotinylated Human PD-L2, His Tag on Anti-His-Biosensor can bind Human PD-1, Llama IgG2b Fc Tag with an affinity constant of 16.3 nM as determined in BLI assay . Loaded Biotinylated Human PD-L2, His Tag on Anti-His-Biosensor can bind Human PD-1, Llama IgG2b Fc Tag with an affinity constant of 16.3 nM as determined in BLI assay .
The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC. The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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