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Human PD-1/PDCD1 Protein, Ultra Low Endotoxin (PD1-HM301-UL) Summary
Product Description |
Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu25-Gln167. |
Product Background |
Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human PD-1/PDCD1 Protein, Ultra Low Endotoxin (PD1-HM301-UL) Specifications/Details
Protein |
PD-1/PDCD1 |
Synonyms |
PDCD1; PD1; CD279; SLEB2; PD-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu25-Gln167 |
Molecular Weight |
The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PD-1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). |
Product Tag |
C-mFc |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human PD-1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). |
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Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. |
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Human PD-1 on Bis-Tris PAGE under reduced condition.The purity is greater than 95%. |
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The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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