Human PD-L1/B7-H1 Protein (PDL-HM11D)
Human PD-L1/B7-H1 Protein (PDL-HM11D)
Human PD-L1/B7-H1 Protein (PDL-HM11D)
Human PD-L1/B7-H1 Protein (PDL-HM11D)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe19-Ile226
Catalog #PDL-HM11D
Regular price $270.00
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Product Description
Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Ile226.
Product Background
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Product Category
Recombinant Protein
Target
PD-L1/B7-H1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Phe19-Ile226
Molecular Weight
The protein has a predicted MW of 24.93 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human PD-L1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.

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