Human PD-L1/B7-H1 Protein (PDL-HM210)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.3μg/ml determined by ELISA. The affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Phe19-Arg238

Catalog: PDL-HM210

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Product Description Recombinant Human PD-L1/B7-H1 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Phe19-Arg238.
Product Category Recombinant Protein
Target PD-L1/B7-H1
Synonyms CD274; PDL1; PD-L1; PD-L1B7 homolog 1; B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Gene/Protein ID Q9NZQ7-1
Species Human
Exact Sequence Phe19-Arg238
Molecular Weight The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.3μg/ml determined by ELISA. The affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay (Biacore T200).
Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.3μg/ml determined by ELISA.
Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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