Human PD-L1/B7-H1 Protein (PDL-HM210)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. The affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Phe19-Arg238

Catalog: PDL-HM210

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Product Description Recombinant Human PD-L1/B7-H1 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Phe19-Arg238.
Product Category Recombinant Protein
Target PD-L1/B7-H1
Synonyms CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Gene/Protein ID Q9NZQ7-1
Species Human
Exact Sequence Phe19-Arg238
Molecular Weight The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. The affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay (Biacore T200).
Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA.
The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.
Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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