Product Description |
Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe19-Arg238. |
Product Background |
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. |
Product Category |
Recombinant Protein |
Target |
PD-L1/B7-H1 |
Synonyms |
CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe19-Arg238 |
Molecular Weight |
The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PD-1, mFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17 ug/ml determined by ELISA (QC Test). The affinity constant of 2.20 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 2.20 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human PD-1, mFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17 ug/ml determined by ELISA (QC Test). |
|
|
Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC. |
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