Human PD-L1/B7-H1 Protein (PDL-HM210)
Human PD-L1/B7-H1 Protein (PDL-HM210)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human PD-1, mFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17 ug/ml determined by ELISA (QC Test). The affinity constant of 2.20 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Phe19-Arg238
Catalog #PDL-HM210
Regular price $240.00
/
Size

Order now, ships by:

Product Description
Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe19-Arg238.
Product Background
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Product Category
Recombinant Protein
Target
PD-L1/B7-H1
Synonyms
CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Phe19-Arg238
Molecular Weight
The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human PD-1, mFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17 ug/ml determined by ELISA (QC Test). The affinity constant of 2.20 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 2.20 uM as determined in SPR assay (Biacore T200).
Immobilized Human PD-1, mFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17 ug/ml determined by ELISA (QC Test).
Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed