Human PD-L1/B7-H1 Protein (PDL-HM310)

  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PD-L1, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe19-Arg238

Catalog: PDL-HM310

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Product Description Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Phe19-Arg238.

Product Background

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Product Category

Recombinant Protein

Target

PD-L1/B7-H1
Synonyms CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Accession

Species

Human

Amino Acid Range

Phe19-Arg238
Molecular Weight The protein has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human PD-L1, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-mFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human PD-L1, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA.
Immobilized Human PD-L1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test).
The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.
Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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