Human PD-L1/B7-H1 Protein (PDL-HM610)

Express System: HEK293
Product Tag: C-Llama Fc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PD-L1, LIama Fc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Phe19-Arg238

Catalog: PDL-HM610

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Product Description	Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with Llama Fc tag at the C-terminus.It contains Phe19-Arg238.
Product Background	B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Product Category	Recombinant Protein

Target	PD-L1/B7-H1
Accession	Q9NZQ7-1
Species	Human
Amino Acid Range	Phe19-Arg238
Molecular Weight	The protein has a predicted MW of 52.50 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human PD-L1, LIama Fc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-Llama Fc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human PD-L1, LIama Fc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA.
	The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.
	Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.