Human PD-L1/B7-H1 Protein (PDL-HM610)
  • Express System: HEK293
  • Product Tag: C-Llama Fc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PD-L1, LIama Fc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe19-Arg238
Catalog #PDL-HM610
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Product Description
 Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with Llama Fc tag at the C-terminus.It contains Phe19-Arg238.
Product Background
 B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Product Category
 Recombinant Protein
Target
 PD-L1/B7-H1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe19-Arg238
Molecular Weight
 The protein has a predicted MW of 52.50 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human PD-L1, LIama Fc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Product Tag
 C-Llama Fc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human PD-L1, LIama Fc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PD-1, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA.
Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.
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