Product Description |
Recombinant Human PD-L2/B7-DC Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Leu20-Thr220. |
Product Background |
PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals. |
Product Category |
Recombinant Protein |
Target |
PD-L2/B7-DC |
Synonyms |
PDL2; PD-L2; Butyrophilin B7-DC; CD273, PDCD1 ligand 2; PDCD1L2; PDCD1LG2; B7DC; bA574F11.2; Btdc |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu20-Thr220 |
Molecular Weight |
The protein has a predicted MW of 48.60 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human PD-L2, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 0.21 uM as determined in SPR assay (Biacore T200). |
|
|
Human PD-L2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human PD-L2 is greater than 95% as determined by SEC-HPLC. |
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