Human PD-L2/B7-DC Protein (PDL-HM402)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.296 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu20-Thr220

Catalog: PDL-HM402

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Product Description Recombinant Human PD-L2/B7-DC Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Thr220.

Product Background

PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.

Product Category

Recombinant Protein

Target

PD-L2/B7-DC
Synonyms PDL2; PD-L2; Butyrophilin B7-DC; CD273, PDCD1 ligand 2; PDCD1L2; PDCD1LG2; B7DC; bA574F11.2; Btdc
Accession

Species

Human

Amino Acid Range

Leu20-Thr220
Molecular Weight The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 0.296 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L2, His Tag with an affinity constant of 0.296 uM as determined in SPR assay (Biacore T200).
The purity of Human PD-L2 is greater than 95% as determined by SEC-HPLC.
Human PD-L2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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