Human PD-L2/B7-DC Protein (PDL-HM402)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.296 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu20-Thr220

Catalog: PDL-HM402

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Product Description Recombinant Human PD-L2/B7-DC Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Leu20-Thr220.
Product Category Recombinant Protein
Target PD-L2/B7-DC
Synonyms PDL2; PD-L2; Butyrophilin B7-DC; CD273, PDCD1 ligand 2; PDCD1L2; PDCD1LG2; B7DC; bA574F11.2; Btdc
Gene/Protein ID Q9BQ51
Species Human
Exact Sequence Leu20-Thr220
Molecular Weight The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.296 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L2, His Tag with an affinity constant of 0.296 μM as determined in SPR assay (Biacore T200).
The purity of Human PD-L2 is greater than 95% as determined by SEC-HPLC.
Human PD-L2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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