Name: Non-biotinylated Human PD-L2/B7-DC Protein (PDL-HM402)
Brand: KactusBio
SKU: PDL-HM402-100UG
Price: 231.0 USD
Availability: InStock

SPR sensorgram showing real-time binding responses at multiple analyte concentrations, indicating strong association and dissociation kinetics of the interaction

Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L2, His Tag with an affinity constant of 0.296 uM as determined in SPR assay (Biacore T200).

Human PD-L2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human PD-L2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.296 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu20-Thr220

Catalog #PDL-HM402

Size

100UG

500UG

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Non-biotinylated Human PD-L2/B7-DC Protein (PDL-HM402) Summary

Product Description	Recombinant Human PD-L2/B7-DC Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Thr220.
Product Background	PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Non-biotinylated Human PD-L2/B7-DC Protein (PDL-HM402) Specifications/Details

Protein	PD-L2/B7-DC
Synonyms	PDL2; PD-L2; Butyrophilin B7-DC; CD273, PDCD1 ligand 2; PDCD1L2; PDCD1LG2; B7DC; bA574F11.2; Btdc
Accession	Q9BQ51
Species	Human
Biotinylated	no
Amino Acid Range	Leu20-Thr220
Molecular Weight	The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.296 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L2, His Tag with an affinity constant of 0.296 uM as determined in SPR assay (Biacore T200).
	Human PD-L2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human PD-L2 is greater than 95% as determined by SEC-HPLC.