Name: Non-biotinylated Human PDGF R alpha/PDGFRA Protein (PFR-HM40A)
Brand: KactusBio
SKU: PFR-HM40A-100UG
Price: 206.8 USD
Availability: InStock

Non-biotinylated Human PDGF R alpha/PDGFRA Protein (PFR-HM40A)

Immobilized Human PDGF R alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.

Human PDGF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human PDGF R alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PDGF R alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln24-Glu524

Catalog #PFR-HM40A

Size

100UG

500UG

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Product Description	Recombinant Human PDGF R alpha/PDGFRA is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Glu524.
Product Background	Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	PDGF R alpha/PDGFRA
Synonyms	PDGFRA; CD140A; PDGFR2; RHEPDGFRA; MGC74795; PDGFR-2
Accession	P16234
Species	Human
Biotinylated	no
Amino Acid Range	Gln24-Glu524
Molecular Weight	The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PDGF R alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human PDGF R alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
	Human PDGF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human PDGF R alpha is greater than 95% as determined by SEC-HPLC.

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