Human PDGF R beta/CD140b Protein (PGF-HM4RB)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PDGF R beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu33-Phe530

Catalog: PGF-HM4RB

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Product Description	Recombinant Human PDGF R beta/CD140b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu33-Phe530.
Product Background	PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.
Product Category	Recombinant Protein

Target	PDGF R beta/CD140b
Synonyms	JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; CD140B; PDGFR-2; PDGFR-β
Gene/Protein ID	P09619
Species	Human
Exact Sequence	Leu33-Phe530
Molecular Weight	The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 78-115 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human PDGF R beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human PDGF R beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.
	The purity of Human PDGF R beta is greater than 95% as determined by SEC-HPLC.
	Human PDGF R beta on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.