Human Peptide Ready HLA-A*11:01&B2M Monomer-Protein (MHC-HM41R)
Human Peptide Ready HLA-A*11:01&B2M Monomer-Protein (MHC-HM41R)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human Peptide Ready HLA-A*11:01&B2M Monomer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305 (HLA-A*11:01) & Ile21-Met119 (B2M)
Catalog #MHC-HM41R
Regular price $310.00
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Product Description

Recombinant Human Peptide Ready HLA-A*11:01&B2M Monomer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*11:01) and Ile21-Met119 (B2M).

Product Background

Peptide Ready HLA-A*11:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*11:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Product Category

Recombinant Protein / Antibody discovery / MHC

Protein

Peptide Ready HLA-A*11:01&B2M Monomer

Synonyms

MHC; HLA-A*11:01; Peptide Ready

MHC Form

Monomer

Allele

HLA-A*11:01

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly25-Thr305 (HLA-A*11:01) & Ile21-Met119 (B2M)

Molecular Weight

The protein has a predicted MW of 48.50 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Peptide Ready HLA-A*11:01&B2M Monomer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Peptide Ready HLA-A*11:01&B2M Monomer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA. Immobilized Human Peptide Ready HLA-A*11:01&B2M Monomer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.
Human Peptide Ready HLA-A*11:01&B2M Monomer-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Peptide Ready HLA-A*11:01&B2M Monomer-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Peptide Ready HLA-A*11:01&B2M Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human Peptide Ready HLA-A*11:01&B2M Monomer is greater than 95% as determined by SEC-HPLC.

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