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Human Peptide Ready HLA-E*01:03&B2M Monomer-Protein, Ultra Low Endotoxin (MHC-HM42R-UL) Summary
Product Description |
Recombinant Human Peptide Ready HLA-E*01:03&B2M Monomer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly22-Thr302(HLA-E*01:03) and Ile21-Met119(B2M). |
Product Background |
HLA-E*01:03&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-E*01:03. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-ready MHCs |
Peptide Ready HLA-E*01:03&B2M Specifications
Protein |
Peptide Ready HLA-E*01:03&B2M |
Synonyms |
MHC; HLA-E*01:03; Peptide Ready |
MHC Form |
Monomer |
Allele |
HLA-E*01:03 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly22-Thr302(HLA-E*01:03) & Ile21-Met119(B2M) |
Molecular Weight |
The protein has a predicted MW of 48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Peptide Ready HLA-E*01:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 100mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Peptide Ready HLA-E*01:03&B2M Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). |
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Human Peptide Ready HLA-E*01:03&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Peptide Ready HLA-E*01:03&B2M Monomer-is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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