Product Description |
Recombinant Human Periostin/OSF-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn22-Gln836. |
Product Background |
Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function. |
Product Category |
Recombinant Protein |
Target |
Periostin/OSF-2 |
Synonyms |
Periostin; PN; OSF-2; POSTN; OSF2; Fasciclin I-like; PDLPOSTN; PNRP11-412K4.1; TRIF52 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asn22-Gln836 |
Molecular Weight |
The protein has a predicted MW of 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Periostin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Periostin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. |
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Human Periostin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Periostin is greater than 95% as determined by SEC-HPLC. |
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