Human Periostin/OSF-2 Protein (OSF-HM102)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Periostin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Asn22-Gln836

Catalog: OSF-HM102

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Product Description	Recombinant Human Periostin/OSF-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn22-Gln836.
Product Background	Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.
Product Category	Recombinant Protein

Target	Periostin/OSF-2
Synonyms	Periostin; PN; OSF-2; POSTN; OSF2; Fasciclin I-like; PDLPOSTN; PNRP11-412K4.1; TRIF52
Gene/Protein ID	Q15063
Species	Human
Exact Sequence	Asn22-Gln836
Molecular Weight	The protein has a predicted MW of 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Periostin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Periostin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA.
	The purity of Human Periostin is greater than 95% as determined by SEC-HPLC.
	Human Periostin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.