Human Periostin/OSF-2 Protein (OSF-HM102)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Periostin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asn22-Gln836

Catalog: OSF-HM102

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Product Description Recombinant Human Periostin/OSF-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn22-Gln836.

Product Background

Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.

Product Category

Recombinant Protein

Target

Periostin/OSF-2
Synonyms Periostin; PN; OSF-2; POSTN; OSF2; Fasciclin I-like; PDLPOSTN; PNRP11-412K4.1; TRIF52
Accession

Species

Human

Amino Acid Range

Asn22-Gln836
Molecular Weight The protein has a predicted MW of 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Periostin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Periostin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
The purity of Human Periostin is greater than 95% as determined by SEC-HPLC.
Human Periostin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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