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Human PGF Protein, Ultra Low Endotoxin (PGF-HM201-UL) Summary
Product Description |
Recombinant Human PGF Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu19-Arg221. |
Product Background |
Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human PGF Protein, Ultra Low Endotoxin (PGF-HM201-UL) Specifications/Details
Protein |
PGF |
Synonyms |
PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu19-Arg221 |
Molecular Weight |
The protein has a predicted MW of 48.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 53.4ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 53.4ng/ml determined by ELISA. |
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Immobilized Human PGF, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PGF Antibody, hFc Tag with the EC50 of 34.1ng/ml determined by ELISA. |
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Human PGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human PGF is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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