• Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Met1-Cys267
Catalog #PHO-HE101-UL
Price
Regular price $36300
Regular price Sale price $36300
/
Size
Avantor VWR Fisher Scientific

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Human PHOSPHO1-Protein, Ultra Low Endotoxin (PHO-HE101-UL) Summary

Product Description

 Recombinant Human PHOSPHO1-Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Cys267.

Product Background

 PHOSPHO1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralisation.

Human PHOSPHO1-Protein, Ultra Low Endotoxin (PHO-HE101-UL) Specifications/Details

Protein
 PHOSPHO1
Synonyms
 Phosphocholine phosphatase-1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-Cys267
Molecular Weight
 The protein has a predicted MW of 30.67 kDa same as Bis-Tris PAGE result.
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 300mM L-Arginine (pH 7.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human PHOSPHO1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human PHOSPHO1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PHOSPHO1-is greater than 95% as determined by SEC-HPLC. The purity of Human PHOSPHO1-is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...