Human PLA2G1B Protein (PLA-HM11B)
Human PLA2G1B Protein (PLA-HM11B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ala23-Ser148
Catalog #PLA-HM11B
Regular price $315.00
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Product Description
Recombinant Human PLA2G1B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Ser148.
Product Background
Phospholipase A2 (PLA2) plays crucial roles in diverse cellular responses, including phospholipid digestion and metabolism, host defense and signal transduction. PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGs) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA).
Product Category
Recombinant Protein
Target
PLA2G1B
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala23-Ser148
Molecular Weight
The protein has a predicted MW of 15.23 kDa. Due to glycosylation, the protein migrates to 18-23 kDa based on Bis-Tris PAGE result.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 10mM Tris, 5mM CaCl2 (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PLA2G1B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PLA2G1B is greater than 95% as determined by SEC-HPLC.

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