Product Description |
Recombinant Human PLA2G1B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Ser148. |
Product Background |
Phospholipase A2 (PLA2) plays crucial roles in diverse cellular responses, including phospholipid digestion and metabolism, host defense and signal transduction. PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGs) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA). |
Product Category |
Recombinant Protein |
Target |
PLA2G1B |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala23-Ser148 |
Molecular Weight |
The protein has a predicted MW of 15.23 kDa. Due to glycosylation, the protein migrates to 18-23 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 10mM Tris, 5mM CaCl2 (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human PLA2G1B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human PLA2G1B is greater than 95% as determined by SEC-HPLC. |
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