Human PLA2G1B Protein (PLA-HM11B)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ala23-Ser148

Catalog: PLA-HM11B

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Product Description Recombinant Human PLA2G1B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Ser148.

Product Background

Phospholipase A2 (PLA2) plays crucial roles in diverse cellular responses, including phospholipid digestion and metabolism, host defense and signal transduction. PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGs) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA).

Product Category

Recombinant Protein

Target

PLA2G1B
Accession

Species

Human

Amino Acid Range

Ala23-Ser148
Molecular Weight The protein has a predicted MW of 15.23 kDa. Due to glycosylation, the protein migrates to 18-23 kDa based on Tris-Bis PAGE result.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 10mM Tris, 5mM CaCl2 (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human PLA2G1B is greater than 95% as determined by SEC-HPLC.
Human PLA2G1B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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