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Human PLA2G1B Protein (PLA-HM21B) Summary
Product Description |
Recombinant Human PLA2G1B Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala23-Ser148. |
Product Background |
Phospholipase A2 (PLA2) plays crucial roles in diverse cellular responses, including phospholipid digestion and metabolism, host defense and signal transduction. PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGs) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human PLA2G1B Protein (PLA-HM21B) Specifications/Details
Protein |
PLA2G1B |
Synonyms |
Phospholipase A2; PLA2G1B; PLA2; PLA2A; PLA2AMGC119834 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala23-Ser148 |
Molecular Weight |
The protein has a predicted MW of 40.9 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human PLA2G1B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human PLA2G1B is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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