Human PLAU/uPA Protein (active form) (PLA-HM101)
Human PLAU/uPA Protein (active form) (PLA-HM101)
Human PLAU/uPA Protein (active form) (PLA-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ser21-Leu431
Catalog #PLA-HM101
Regular price $300.00
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Product Description
Recombinant Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-Terminus fragment (Ser21-Lys155).
Product Background
Plasminogen activator, urokinase (uPA) is a secreted serine protease, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin. Dysregulation of PLAU is often accompanied by various cancers and PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Product Category
Recombinant Protein
Target
PLAU/uPA
Synonyms
PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser21-Leu431
Molecular Weight
The protein has a predicted MW of 17.9 kDa (long chain A), 29.2 kDa (chain B) and 15.3 kDa (N-terminal fragment). The protein migrates to 30-38 kDa (chain B) and 17 kDa (N-terminal fragment) based on Bis-Tris PAGE result.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 1% HCOOH, 1mM DTT (pH 3.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 1% HCOOH, 1mM DTT (pH 3.0).
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PLAU (activated by trypsin) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PLAU (activated by trypsin) is greater than 95% as determined by SEC-HPLC.

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