Human PLAU/uPA Protein (active form) (PLA-HM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PLAU, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). The affinity constant of 5.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser21-Leu431

Catalog: PLA-HM101

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Product Description Recombinant Human PLAU/uPA Protein (active form) is expressed from Expi293 with His tag at the C-terminal.It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-terminal fragment (Ser21-Lys155).
Product Category Recombinant Protein
Target PLAU/uPA
Synonyms PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
Gene/Protein ID P00749-1
Species Human
Exact Sequence Ser21-Leu431
Molecular Weight The protein has a predicted MW of 47.5 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa(long chain A), 35-38 kDa(chain B) and 52-60 kDa(long chain A&chain B) based on Tris-Bis PAGE result.
Activity Immobilized Human PLAU, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). The affinity constant of 5.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse uPAR isoform 1, His Tag immobilized on CM5 Chip can bind Human PLAU, His Tag with an affinity constant of 5.87 nM as determined in SPR assay (Biacore T200).
Immobilized Human PLAU, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test).
The purity of Human PLAU (activated by trypsin) is greater than 95% as determined by SEC-HPLC.
Human PLAU (activated by trypsin) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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