Human PLAU/uPA Protein (pro form)  (PLA-HM102)
Human PLAU/uPA Protein (pro form)  (PLA-HM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PLAU, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser21-Leu431
Catalog #PLA-HM102
Regular price $330.00
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Product Description

Recombinant Human PLAU/uPA Protein (pro form) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Leu431.

Product Background

Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
PLAU/uPA
Synonyms
PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser21-Leu431
Molecular Weight
The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human PLAU, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human PLAU, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. Immobilized Human PLAU, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Human PLAU (pro form) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human PLAU (pro form) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PLAU (pro form) is greater than 95% as determined by SEC-HPLC. The purity of Human PLAU (pro form) is greater than 95% as determined by SEC-HPLC.
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