Human PLAU/uPA Protein (pro form) (PLA-HM102)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PLAU, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser21-Leu431

Catalog: PLA-HM102

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Product Description	Recombinant Human PLAU/uPA Protein (pro form) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Leu431.
Product Background	Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Product Category	Recombinant Protein

Target	PLAU/uPA
Synonyms	PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
Gene/Protein ID	P00749-1
Species	Human
Exact Sequence	Ser21-Leu431
Molecular Weight	The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human PLAU, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human PLAU, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
	The purity of Human PLAU (pro form) is greater than 95% as determined by SEC-HPLC.
	Human PLAU (pro form) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.