Product Description |
Recombinant Human PLD4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp52-Gly506. |
Product Background |
Phospholipase D4 (PLD4) is a newly identified protein expressed in microglia. the expression of PLD4 was located in macrophages in the colon cancer mesenchymal and lymph nodes as shown by immunohistochemical analysis. furthermore, its expression was associated with clinical staging of colon cancer. Then, THP-1 as a cell model induced into TAMs. PLD4 could be involved in the activation process of M1 phenotype macrophages. |
Product Category |
Recombinant Protein |
Protein |
PLD4 |
Synonyms |
5'-3' exonuclease PLD4; Choline phosphatase 4; Phospholipase D4; PLD 4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Trp52-Gly506 |
Molecular Weight |
The protein has a predicted MW of 51.1 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM MES, 100mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 100mM NaCl (pH 6.0). |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human PLD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human PLD4 is greater than 95% as determined by SEC-HPLC. |
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