Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein (MHC-HM447)
Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein (MHC-HM447)
Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein (MHC-HM447)
Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein (MHC-HM447)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 79.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide
Catalog #MHC-HM447
Regular price $341.00
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Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein (MHC-HM447) Summary

Product Description

Recombinant Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide.

Product Background

PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Specifications

Protein
HLA-A*02:01&B2M&PRAME (ALYVDSLFFL)
Synonyms
PRAME; OIP-4; OIP4; MAPE;

MHC Form

Monomer

Allele

HLA-A*02:01
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide
Molecular Weight
The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 79.5ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 79.5ng/ml determined by ELISA (QC Test). Immobilized Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 79.5ng/ml determined by ELISA (QC Test).
Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer is greater than 95% as determined by SEC-HPLC.
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