Name: Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein (MHC-HM447)
Brand: KactusBio
SKU: MHC-HM447-100UG
Price: 310.0 USD
Availability: InStock

Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein (MHC-HM447)

Immobilized Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 79.5ng/ml determined by ELISA (QC Test).

Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 79.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide

Catalog #MHC-HM447

Size

100UG

500UG

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Product Description	Recombinant Human HLA-A02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide.
Product Background	PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer
Synonyms	PRAME; OIP-4; OIP4; MAPE;
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	P79603(HLA-A*02:01)&P61769(B2M)&ALYVDSLFFL
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide
Molecular Weight	The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human HLA-A02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 79.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human HLA-A02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 79.5ng/ml determined by ELISA (QC Test).
	Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer is greater than 95% as determined by SEC-HPLC.