Human PRLR Protein (PLR-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PRLR, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.96nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln25-Asp234

Catalog: PLR-HM101

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Product Description	Recombinant Human PRLR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-Asp234.
Product Category	Recombinant Protein

Target	PRLR
Synonyms	PRL-R; HPRL; MFAB; Prolactin R
Gene/Protein ID	P16471-1
Species	Human
Exact Sequence	Gln25-Asp234
Molecular Weight	The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human PRLR, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.96nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM Tris, 150nM Nacl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human PRLR, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-PRLR Antibody, hFc Tag with an affinity constant of 0.96nM as determined in SPR assay (Biacore T200).
	Immobilized Human PRLR, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test).
	The purity of Human PRLR is greater than 95% as determined by SEC-HPLC.
	Human PRLR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.