Human PRLR Protein (PLR-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PRLR, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.96nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln25-Asp234

Catalog: PLR-HM101

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Product Description Recombinant Human PRLR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-Asp234.

Product Background

Prolactin receptor (PRLR) is highly expressed in a subset of human breast cancer and prostate cancer, which makes it a potential target for cancer treatment. In clinical trials, the blockade of PRLR was shown to be safe but with poor efficacy. It is therefore urgent to develop new therapies against PRLR target. Bispecific antibodies (BsAbs) could guide immune cells toward tumor cells, and produced remarkable effects in some cancers.

Product Category

Recombinant Protein

Target

PRLR
Synonyms PRL-R; HPRL; MFAB; Prolactin R
Accession

Species

Human

Amino Acid Range

Gln25-Asp234
Molecular Weight The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human PRLR, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.96nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM Tris, 150nM Nacl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PRLR, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-PRLR Antibody, hFc Tag with an affinity constant of 0.96nM as determined in SPR assay (Biacore T200).
Immobilized Human PRLR, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test).
The purity of Human PRLR is greater than 95% as determined by SEC-HPLC.
Human PRLR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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