Human PRNP Protein (PRP-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human PRNP, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 33.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Lys23-Ser230

Catalog: PRP-HM201

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Product Description	Recombinant Human PRNP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys23-Ser230.
Product Background	Prion protein gene (PRNP) variants determine the susceptibility of humans, sheep and mice to prion diseases, whereas polymorphisms in the open reading frame (ORF) of bovine PRNP seem to be unrelated to the incidence of bovine spongiform encephalopathy (BSE). According to the latest reports, the genetic susceptibility of cattle to BSE is associated with polymorphisms ofthe regulatory region of the PRNP gene and the level ofits expression.
Product Category	Recombinant Protein

Target	PRNP
Synonyms	Major prion protein；PrP；ALTPRP；PRIP；PRP；PRNP
Gene/Protein ID	P04156
Species	Human
Exact Sequence	Lys23-Ser230
Molecular Weight	The protein has a predicted MW of 49.4 kDa. Due to glycosylation, the protein migrates to 60-67 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human PRNP, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 33.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human PRNP, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 33.2ng/ml determined by ELISA.
	Human PRNP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.