Product Description |
Recombinant Human PRNP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys23-Ser230. |
Product Background |
Prion protein gene (PRNP) variants determine the susceptibility of humans, sheep and mice to prion diseases, whereas polymorphisms in the open reading frame (ORF) of bovine PRNP seem to be unrelated to the incidence of bovine spongiform encephalopathy (BSE). According to the latest reports, the genetic susceptibility of cattle to BSE is associated with polymorphisms ofthe regulatory region of the PRNP gene and the level ofits expression. |
Product Category |
Recombinant Protein |
Target |
PRNP |
Synonyms |
Major prion protein;PrP;ALTPRP;PRIP;PRP;PRNP |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys23-Ser230 |
Molecular Weight |
The protein has a predicted MW of 49.4 kDa. Due to glycosylation, the protein migrates to 60-67 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PRNP, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 41.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human PRNP, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 41.7ng/ml determined by ELISA. |
|
|
Human PRNP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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