Name: Human pro GDF-8 Protein (GDF-HM118)
Brand: KactusBio
SKU: GDF-HM118-100UG
Price: 450.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human pro GDF-8, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Apitegromab, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA.
Amino Acid Range: Asn24-Ser375
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Catalog #GDF-HM118

Size

100UG

500UG

Order by 4pm ET for delivery on:

Human pro GDF-8 Protein (GDF-HM118) Summary

Product Description	Recombinant Human pro GDF-8 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Asn24-Ser375.
Product Background	Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human pro GDF-8 Protein (GDF-HM118) Specifications/Details

Protein	GDF-8
Synonyms	Myostatin; GDF8; Mstn; GDF-8; Myostatin
Accession	O14793
Species	Human
Biotinylated	no
Amino Acid Range	Asn24-Ser375
Molecular Weight	The protein has a predicted MW of 41.19 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human pro GDF-8, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Apitegromab, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human pro GDF-8, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Apitegromab, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA.
	Immobilized Human pro GDF-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 35.9ng/ml determined by ELISA.
	Human pro GDF-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Apitegromab, hFc Tag on ProA-Biosensor can bind Human pro GDF-8, His Tag with an affinity constant of 25.10nM as determined in BLI assay (Gator® Prime).
	The purity of Human pro GDF-8 is greater than 95% as determined by SEC-HPLC.