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Human PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-HM210-UL) Summary
Product Description |
Recombinant Human PSMA/FOLH1 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Lys44-Ala750. |
Product Background |
Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets |
Human PSMA/FOLH1 Protein (active dimer), Ultra Low Endotoxin (PSM-HM210-UL) Specifications/Details
Protein |
PSMA/FOLH1 |
Synonyms |
GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys44-Ala750 |
Molecular Weight |
The protein has a predicted MW of 106.8 kDa. Due to glycosylation, the protein migrates to 120-150 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PSMA, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PSMA Antibody, hFc Tag with the EC50 of 29.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >500 pmol/min/µg. |
Product Tag |
N-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl, 8% trehalose (pH 6.0). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human PSMA, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PSMA Antibody, hFc Tag with the EC50 of 29.9ng/ml determined by ELISA. |
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Human PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human PSMA is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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