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Human PTK7/CCK4 Protein, Ultra Low Endotoxin (CCK-HM204-UL) Summary
Product Description |
Recombinant Human PTK7/CCK4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ala31-Thr704. |
Product Background |
Protein Tyrosine Kinase 7 (PTK7) is as a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. Observations in different model systems suggest a complex cross-talk between PTK7 protein and Wnt signaling. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human PTK7/CCK4 Protein, Ultra Low Endotoxin (CCK-HM204-UL) Specifications/Details
Protein |
PTK7/CCK4 |
Synonyms |
CCK-4; PTK7 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala31-Thr704 |
Molecular Weight |
The protein has a predicted MW of 100.56 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PTK7, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PTK7 Antibody, hFc Avi Tag with the EC50 of 24.2ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human PTK7, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PTK7 Antibody, hFc Avi Tag with the EC50 of 24.2ng/ml determined by ELISA. |
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Human PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human PTK7 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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