Human PVRIG Protein (PVR-HM302)
Human PVRIG Protein (PVR-HM302)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test). The affinity constant of 87.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr41-Asp171
Catalog #PVR-HM302
Regular price $330.00
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Product Description

Recombinant Human PVRIG Protein is expressed from HEK293 with mFc (IgG2a) tag at the C-terminus.It contains Thr41-Asp171.

Product Background

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

PVRIG

Synonyms

C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Thr41-Asp171

Molecular Weight

The protein has a predicted MW of 40.03 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test). The affinity constant of 87.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-mFc

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200). Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200).
Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test). Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test).
Immobilized Human PVRIG, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. Immobilized Human PVRIG, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
Human PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PVRIG is greater than 95% as determined by SEC-HPLC. The purity of Human PVRIG is greater than 95% as determined by SEC-HPLC.

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