Human PVRIG Protein (PVR-HM302)

Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200).

Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test).

Immobilized Human PVRIG, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.

Human PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human PVRIG is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-mFc
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test). The affinity constant of 87.49 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Thr41-Asp171

Catalog #PVR-HM302

Price

$363⁰⁰

Size

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Human PVRIG Protein (PVR-HM302) Summary

Product Description

Recombinant Human PVRIG Protein is expressed from HEK293 with mFc (IgG2a) tag at the C-terminus.It contains Thr41-Asp171.

Product Background

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

Human PVRIG Protein (PVR-HM302) Specifications/Details

Protein	PVRIG
Synonyms	C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1;RalGDS/AF6
Accession	Q6DKI7
Species	Human
Biotinylated	no
Amino Acid Range	Thr41-Asp171
Molecular Weight	The protein has a predicted MW of 40.03 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test). The affinity constant of 87.49 nM as determined in SPR assay (Biacore T200).
Product Tag	C-mFc
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200).
	Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test).
	Immobilized Human PVRIG, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
	Human PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human PVRIG is greater than 90% as determined by SEC-HPLC.