Human R spondin 1/RSPO1 Protein (RS1-HM101)

  • Express System: CHO
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human R spondin 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Arg31-Ala263

Catalog: RS1-HM101

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Product Description Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-terminal.It contains Arg31-Ala263.
Product Category Recombinant Protein
Target R spondin 1/RSPO1
Synonyms R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO
Gene/Protein ID Q2MKA7
Species Human
Exact Sequence Arg31-Ala263
Molecular Weight The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human R spondin 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag C-His
Express System CHO
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human R spondin 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA (QC Test).
Immobilized Human R spondin 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4μg/ml determined by ELISA.
Human R spondin 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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