Name: Human R spondin 1/RSPO1 Protein (RS1-HM101)
Brand: KactusBio
SKU: RS1-HM101-100UG
Price: 198.0 USD
Availability: InStock

ELISA binding curve of human R spondin 1 His tag protein showing mean absorbance versus log anti-R spondin 1 antibody concentration

Human R spondin 1/RSPO1 Protein (RS1-HM101)

Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).

Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA.

Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human R spondin 1, His Tag with the EC50 of 4.4ug/ml determined by ELISA.

Human R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50-for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

Express System: CHO
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Arg31-Ala263

Catalog #RS1-HM101

Size

100UG

500UG

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Product Description	Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg31-Ala263.
Product Background	The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	R spondin 1/RSPO1
Synonyms	R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1
Accession	Q2MKA7
Species	Human
Biotinylated	no
Amino Acid Range	Arg31-Ala263
Molecular Weight	The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	CHO
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.05 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
	Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA.
	Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human R spondin 1, His Tag with the EC50 of 4.4ug/ml determined by ELISA.
	Human R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50-for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

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