Human R spondin 1/RSPO1 Protein (RS1-HM101)
Human R spondin 1/RSPO1 Protein (RS1-HM101)
Human R spondin 1/RSPO1 Protein (RS1-HM101)
Human R spondin 1/RSPO1 Protein (RS1-HM101)
  • Express System: CHO
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Arg31-Ala263
Catalog #RS1-HM101
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Product Description
 Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg31-Ala263.
Product Background
 The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Product Category
 Recombinant Protein
Target
 R spondin 1/RSPO1
Synonyms
 R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Arg31-Ala263
Molecular Weight
 The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His
Express System
 CHO
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 0.05EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA.
Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human R spondin 1, His Tag with the EC50 of 4.4ug/ml determined by ELISA.
Human R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50-for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

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