Human R spondin 1/RSPO1 Protein (RS1-HM101)

  • Express System: CHO
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human R spondin 1, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg31-Ala263

Catalog: RS1-HM101

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Product Description Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-terminal.It contains Arg31-Ala263.
Product Category Recombinant Protein
Target R spondin 1/RSPO1
Synonyms R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO
Gene/Protein ID Q2MKA7
Species Human
Exact Sequence Arg31-Ala263
Molecular Weight The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human R spondin 1, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System CHO
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human R spondin 1, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
Immobilized Human R spondin 1, His Tag at 5_g/ml (100_l/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4_g/ml determined by ELISA.
Human R spondin 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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