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Human R spondin 1/RSPO1 Protein, Ultra Low Endotoxin (RS1-HM101-UL) Summary
Product Description |
Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg31-Ala263. |
Product Background |
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human R spondin 1/RSPO1 Protein, Ultra Low Endotoxin (RS1-HM101-UL) Specifications/Details
Protein |
R spondin 1/RSPO1 |
Synonyms |
R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg31-Ala263 |
Molecular Weight |
The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
CHO |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). |
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Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA. |
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Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human R spondin 1, His Tag with the EC50 of 4.4ug/ml determined by ELISA. |
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Human R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50-for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test). |
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Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind Human RNF43, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay . |
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Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind Human LRP-6, mFc Tag with an affinity constant of 8.97 nM as determined in BLI assay . |
Other Available Proteins:
Finding available proteins...
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