Human RANKL/TNFSF11/CD254 Protein (RKL-HM001)
Catalog: RKL-HM001
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Product Description | Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 without tag.It contains Gly63-Asp244. |
Product Background |
Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone. |
Product Category |
Recombinant Protein |
Target |
RANKL/TNFSF11/CD254 |
Synonyms | ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2 |
Gene/Protein ID |
O14788-2 |
Species |
Human |
Exact Sequence |
Gly63-Asp244 |
Molecular Weight | The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Tris-Bis PAGE resu |
Activity |
Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity | > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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