Human RANKL/TNFSF11/CD254 Protein (RKL-HM001)

  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly63-Asp244

Catalog: RKL-HM001

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Product Description Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 without tag.It contains Gly63-Asp244.

Product Background

Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.

Product Category

Recombinant Protein

Target

RANKL/TNFSF11/CD254
Synonyms ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2

Gene/Protein ID

O14788-2

Species

Human

Exact Sequence

Gly63-Asp244
Molecular Weight The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Tris-Bis PAGE resu

Activity

Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

No Tag

Express System

HEK293
Purity > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test).
The purity of Human RANKL is greater than 90% as determined by SEC-HPLC.
Human RANKL on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

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