Human RANKL/TNFSF11/CD254 Protein (RKL-HM001)

Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).

Immobilized Human RANKL, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test).

Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.2ng/ml determined by ELISA.

Human RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human RANKL is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human RANKL, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly63-Asp244

Catalog #RKL-HM001

Price

$412⁵⁰

Size

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Human RANKL/TNFSF11/CD254 Protein (RKL-HM001) Summary

Product Description

Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 without tag.It contains Gly63-Asp244.

Product Background

Receptor activator of nuclear factor κB (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.

Human RANKL/TNFSF11/CD254 Protein (RKL-HM001) Specifications/Details

Protein	RANKL/TNFSF11/CD254
Synonyms	ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2
Accession	O14788-2
Species	Human
Biotinylated	no
Amino Acid Range	Gly63-Asp244
Molecular Weight	The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Bis-Tris PAGE resu
Activity	Immobilized Human RANKL, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Product Tag	No Tag
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
	Immobilized Human RANKL, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test).
	Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.2ng/ml determined by ELISA.
	Human RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human RANKL is greater than 95% as determined by SEC-HPLC.