Human RANKL/TNFSF11/CD254 Protein (RKL-HM101)

  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 90% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly63-Asp244

Catalog: RKL-HM101

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Product Description Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gly63-Asp244.

Product Background

Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.

Product Category

Recombinant Protein

Target

RANKL/TNFSF11/CD254
Synonyms ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2
Accession

Species

Human

Amino Acid Range

Gly63-Asp244
Molecular Weight The protein has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Flag

Express System

HEK293
Purity > 90% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
Human Rankl on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

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