Product Description |
Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gly63-Asp244. |
Product Background |
Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone. |
Product Category |
Recombinant Protein |
Target |
RANKL/TNFSF11/CD254 |
Synonyms |
ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly63-Asp244 |
Molecular Weight |
The protein has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Flag |
Express System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. |
|
|
Human Rankl on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
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