Human RGM-B Protein (RGM-HM10B)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Gly46-Asn413

Catalog #RGM-HM10B

Price

$330⁰⁰

Size

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Human RGM-B Protein (RGM-HM10B) Summary

Product Description

Recombinant Human RGM-B Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly46-Asn413.

Product Background

RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.

Human RGM-B Protein (RGM-HM10B) Specifications/Details

Protein	RGM-B
Synonyms	RGMB; DRAGON;
Accession	Q6NW40
Species	Human
Biotinylated	no
Amino Acid Range	Gly46-Asn413
Molecular Weight	The protein has a predicted MW of 41.88 kDa. Due to autocatalytic cleavage, the protein migrates to 20 kDa and 33-40 kDa based on Bis-Tris PAGE result.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human RGM-B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human RGM-B is greater than 95% as determined by SEC-HPLC.