Human RGM-C Domain Protein (RGM-HM30D)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human RGM-C Domain, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln36-Pro145
Catalog #RGM-HM30D
Regular price $345.00
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Product Description
Recombinant Human RGM-C Domain Protein is expressed from HEK293 with mFc (IgG2a) tag at the C-terminus.It contains Gln36-Pro145.
Product Background
RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.
Product Category
Recombinant Protein
Target
RGM-C Domain
Synonyms
Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln36-Pro145
Molecular Weight
The protein has a predicted MW of 37.43 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human RGM-C Domain, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-mFc
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human RGM-C Domain, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).
Human RGM-C Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human RGM-C Domain is greater than 95% as determined by SEC-HPLC.

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