Human RGM-C Protein (RGM-HM10C)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human RGM-C, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln33-Asp393

Catalog: RGM-HM10C

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Product Description	Recombinant Human RGM-C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln33-Asp393.
Product Background	RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.
Product Category	Recombinant Protein

Target	RGM-C
Synonyms	Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C
Gene/Protein ID	Q6ZVN8-1
Species	Human
Exact Sequence	Gln33-Asp393
Molecular Weight	The protein has a predicted MW of 40 kDa (mature)&26 kDa (C-terminus peptide)&14 kDa (N-terminus peptide). Due to enzyme lysis and glycosylation, the protein migrates to 55-60 kDa&30-40 kDa&20-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human RGM-C, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human RGM-C, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
	The purity of Human RGM-C is greater than 95% as determined by SEC-HPLC.
	Human RGM-C on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.