Product Description |
Recombinant Human RGM-C Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln36-Asp400. |
Product Background |
RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system. |
Product Category |
Recombinant Protein |
Target |
RGM-C |
Synonyms |
Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln36-Asp400 |
Molecular Weight |
The protein has a predicted MW of 40 kDa (mature), 26 kDa (C-terminus peptide) and 14 kDa (N-terminus peptide). Due to enzyme lysis and glycosylation, the protein migrates to 55-60 kDa, 30-40 kDa, 20-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. |
|
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Human RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human RGM-C is greater than 95% as determined by SEC-HPLC. |
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