Human RGM-C Protein (RGM-HM10C)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln33-Asp393

Catalog: RGM-HM10C

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Product Description Recombinant Human RGM-C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln33-Asp393.

Product Background

RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.

Product Category

Recombinant Protein

Target

RGM-C
Synonyms Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C
Accession

Species

Human

Amino Acid Range

Gln33-Asp393
Molecular Weight The protein has a predicted MW of 40 kDa (mature)&26 kDa (C-terminus peptide)&14 kDa (N-terminus peptide). Due to enzyme lysis and glycosylation, the protein migrates to 55-60 kDa&30-40 kDa&20-25 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
The purity of Human RGM-C is greater than 95% as determined by SEC-HPLC.
Human RGM-C on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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