Human RGM-C Protein (RGM-HM20C)

Immobilized Human RGM-C, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.

Human RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human RGM-C is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human RGM-C, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Amino Acid Range: Gln36-Asp400

Catalog #RGM-HM20C

Price

$345⁰⁰

Size

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Human RGM-C Protein (RGM-HM20C) Summary

Product Description

Recombinant Human RGM-C Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Gln36-Asp400.

Product Background

RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.

Human RGM-C Protein (RGM-HM20C) Specifications/Details

Protein	RGM-C
Synonyms	Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C
Accession	Q6ZVN8-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln36-Asp400
Molecular Weight	The protein has a predicted MW of 64.81 kDa. Due to autocatalytic cleavage, the protein migrates to 80-90 kDa (full length), 60-70 kDa (C terminal chain) and 22-27 kDa (N terminal chain) based on Bis-Tris PAGE result.
Activity	Immobilized Human RGM-C, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human RGM-C, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
	Human RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human RGM-C is greater than 95% as determined by SEC-HPLC.