Product Description |
Recombinant Human RGMa Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys48-Gly422. |
Product Background |
Repulsive guidance molecule (RGM) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that has diverse functions in the developing and pathological central nervous system (CNS). The binding of RGM to its receptor neogenin regulates axon guidance, neuronal differentiation, and survival during the development of the CNS. RGMa induces T cell activation in experimental autoimmune encephalomyelitis (EAE), which is the animal model of multiple sclerosis (MS).- RGM is expressed in pathogenic Th17 cells and induces neurodegeneration by binding to neogenin. |
Product Category |
Recombinant Protein |
Target |
RGMa |
Synonyms |
RGMA; RGM |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys48-Gly422 |
Molecular Weight |
The protein has a predicted MW of 44.5 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. |
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Human RGMa on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human RGMa is greater than 95% as determined by SEC-HPLC. |
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