Name: Non-biotinylated Human RGMa Protein (RGM-HM401)
Brand: KactusBio
SKU: RGM-HM401-100UG
Price: 297.0 USD
Availability: InStock

ELISA binding curve showing dose-dependent interaction between immobilized human RGMa, His tag, and human Neogenin, hFc tag, with absorbance measured at OD450.

Non-biotinylated Human RGMa Protein (RGM-HM401)

Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).

Human RGMa on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human RGMa is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
Amino Acid Range: Cys48-Gly422

Catalog #RGM-HM401

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Non-biotinylated Human RGMa Protein (RGM-HM401) Summary

Product Description	Recombinant Human RGMa Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys48-Gly422.
Product Background	Repulsive guidance molecule (RGM) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that has diverse functions in the developing and pathological central nervous system (CNS). The binding of RGM to its receptor neogenin regulates axon guidance, neuronal differentiation, and survival during the development of the CNS. RGMa induces T cell activation in experimental autoimmune encephalomyelitis (EAE), which is the animal model of multiple sclerosis (MS).- RGM is expressed in pathogenic Th17 cells and induces neurodegeneration by binding to neogenin.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Non-biotinylated Human RGMa Protein (RGM-HM401) Specifications/Details

Protein	RGMa
Synonyms	RGMA; RGM
Accession	Q96B86-1
Species	Human
Biotinylated	no
Amino Acid Range	Cys48-Gly422
Molecular Weight	The protein has a predicted MW of 44.5 kDa. Due to autocatalytic cleavage, the protein migrates to 27-35 kDa and 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
	Human RGMa on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human RGMa is greater than 95% as determined by SEC-HPLC.

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Non-biotinylated Human RGMa Protein (RGM-HM401)

Non-biotinylated Human RGMa Protein (RGM-HM401)

Non-biotinylated Human RGMa Protein (RGM-HM401) Summary

Product Description

Product Background

Product Category

Non-biotinylated Human RGMa Protein (RGM-HM401) Specifications/Details

Fluorophore

Shipping, Reconstitution, & Storage

Validation Images

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