Human RNF43 Protein (RNF-HM234)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Gly24-Tyr197

Catalog: RNF-HM234

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Product Description Recombinant Human RNF43 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Gly24-Tyr197.
Product Category Recombinant Protein
Target RNF34
Synonyms E3 ubiquitin-protein ligase RNF43；RING finger protein 43；RING-type E3 ubiquitin transferase RNF43；RNF43
Gene/Protein ID Q68DV7
Species Human
Exact Sequence Gly24-Tyr197
Molecular Weight The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag C-hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test).
Immobilized Human R spondin 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4μg/ml determined by ELISA.
The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.
Human RNF43 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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