Name: Human RNF43 Protein (RNF-HM234)
Brand: KactusBio
SKU: RNF-HM234-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly24-Tyr197

Catalog #RNF-HM234

Size

100UG

500UG

Order now, ships by:

Product Description	Recombinant Human RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Tyr197.
Product Background	RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	RNF43
Synonyms	E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43
Accession	Q68DV7-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly24-Tyr197
Molecular Weight	The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).
	Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA.
	Human RNF43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.