Human RNF43 Protein (RNF-HM234)
Catalog: RNF-HM234
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Product Description | Recombinant Human RNF43 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Gly24-Tyr197. |
Product Category | Recombinant Protein |
Target | RNF34 |
Synonyms | E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43 |
Gene/Protein ID | Q68DV7 |
Species | Human |
Exact Sequence | Gly24-Tyr197 |
Molecular Weight | The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag | C-hFc |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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