Human RNF43 Protein (RNF-HM234)
Human RNF43 Protein (RNF-HM234)
Human RNF43 Protein (RNF-HM234)
Human RNF43 Protein (RNF-HM234)
Human RNF43 Protein (RNF-HM234)
Human RNF43 Protein (RNF-HM234)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly24-Tyr197
Catalog #RNF-HM234
/
Size

Order now, ships by:

Product Description
 Recombinant Human RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Tyr197.
Product Background
 RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
Product Category
 Recombinant Protein
Target
 RNF34
Synonyms
 E3 ubiquitin-protein ligase RNF43；RING finger protein 43；RING-type E3 ubiquitin transferase RNF43；RNF43
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly24-Tyr197
Molecular Weight
 The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA.
Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).
Human RNF43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.
Recently viewed