Human ROBO4 Protein (ROB-HM104)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ROBO4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala27-Glu469

Catalog: ROB-HM104

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Product Description Recombinant Human ROBO4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Glu469.

Product Background

Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis.

Product Category

Recombinant Protein

Target

ROBO4
Synonyms ECSM4; MRB; FLJ20798; MGC133352; MGC133353; ROBO4
Accession

Species

Human

Amino Acid Range

Ala27-Glu469
Molecular Weight The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human ROBO4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ROBO4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA.
The purity of Human ROBO4 is greater than 95% as determined by SEC-HPLC.
Human ROBO4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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