Human ROBO4 Protein (ROB-HM104)
Human ROBO4 Protein (ROB-HM104)
Human ROBO4 Protein (ROB-HM104)
Human ROBO4 Protein (ROB-HM104)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ROBO4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala27-Glu469
Catalog #ROB-HM104
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Product Description
 Recombinant Human ROBO4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Glu469.
Product Background
 Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis.
Product Category
 Recombinant Protein
Target
 ROBO4
Synonyms
 ECSM4; MRB; FLJ20798; MGC133352; MGC133353; ROBO4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Glu469
Molecular Weight
 The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ROBO4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ROBO4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA.
Human ROBO4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ROBO4 is greater than 95% as determined by SEC-HPLC.
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