Name: Human ROBO4 Protein (ROB-HM104)
Brand: KactusBio
SKU: ROB-HM104-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ROBO4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala27-Glu469

Catalog #ROB-HM104

Size

100UG

500UG

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Human ROBO4 Protein (ROB-HM104) Summary

Product Description	Recombinant Human ROBO4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Glu469.
Product Background	Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human ROBO4 Protein (ROB-HM104) Specifications/Details

Protein	ROBO4
Synonyms	ECSM4; MRB; FLJ20798; MGC133352; MGC133353; ROBO4
Accession	Q8WZ75-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala27-Glu469
Molecular Weight	The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ROBO4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human ROBO4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
	Human ROBO4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human ROBO4 is greater than 95% as determined by SEC-HPLC.