Human SECTM1 Protein (SEC-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln29-Gly145

Catalog: SEC-HM201

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Product Description Recombinant Human SECTM1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln29-Gly145.

Product Background

SECTM1 is a T/NK cell "co-stimulatory" molecule that is expressed in the peripheral blood by neutrophils and monocytes.Human monocytic cells also displayed a pronounced negative regulation of SECTM1 mRNA expression by LPS, while at the protein level SECTM1 expression was also shown to be regulated by IFN and LPS. This tight regulation of SECTM1 gene expression and rapid upregulation highlights its relevance in the innate immune response.

Product Category

Recombinant Protein

Target

SECTM1
Synonyms K-12; SECTM1
Accession

Species

Human

Amino Acid Range

Gln29-Gly145
Molecular Weight The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 47-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
The purity of Human SECTM1 is greater than 95% as determined by SEC-HPLC.
Human SECTM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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