Human SECTM1 Protein (SEC-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln29-Gly145

Catalog: SEC-HM201

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Product Description	Recombinant Human SECTM1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln29-Gly145.
Product Background	SECTM1 is a T/NK cell "co-stimulatory" molecule that is expressed in the peripheral blood by neutrophils and monocytes.Human monocytic cells also displayed a pronounced negative regulation of SECTM1 mRNA expression by LPS, while at the protein level SECTM1 expression was also shown to be regulated by IFN and LPS. This tight regulation of SECTM1 gene expression and rapid upregulation highlights its relevance in the innate immune response.
Product Category	Recombinant Protein

Target	SECTM1
Synonyms	K-12; SECTM1
Gene/Protein ID	AAH17716
Species	Human
Exact Sequence	Gln29-Gly145
Molecular Weight	The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 47-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
	The purity of Human SECTM1 is greater than 95% as determined by SEC-HPLC.
	Human SECTM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.