Product Description |
Recombinant Human SECTM1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln29-Gly145. |
Product Background |
SECTM1 is a T/NK cell "co-stimulatory" molecule that is expressed in the peripheral blood by neutrophils and monocytes.Human monocytic cells also displayed a pronounced negative regulation of SECTM1 mRNA expression by LPS, while at the protein level SECTM1 expression was also shown to be regulated by IFN and LPS. This tight regulation of SECTM1 gene expression and rapid upregulation highlights its relevance in the innate immune response. |
Product Category |
Recombinant Protein |
Target |
SECTM1 |
Synonyms |
K-12; SECTM1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln29-Gly145 |
Molecular Weight |
The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 47-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 4.46 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human SECTM1, hFc Tag captured on CM5 Chip via Protein A can bind Mouse CD7, His Tag with an affinity constant of 4.46 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). |
|
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Human SECTM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human SECTM1 is greater than 95% as determined by SEC-HPLC. |
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