Name: Human SECTM1 Protein (SEC-HM201)
Brand: KactusBio
SKU: SEC-HM201-100UG
Price: 214.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 4.46 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gln29-Gly145

Catalog #SEC-HM201

Size

100UG

500UG

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Human SECTM1 Protein (SEC-HM201) Summary

Product Description	Recombinant Human SECTM1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln29-Gly145.
Product Background	SECTM1 is a T/NK cell "co-stimulatory" molecule that is expressed in the peripheral blood by neutrophils and monocytes.Human monocytic cells also displayed a pronounced negative regulation of SECTM1 mRNA expression by LPS, while at the protein level SECTM1 expression was also shown to be regulated by IFN and LPS. This tight regulation of SECTM1 gene expression and rapid upregulation highlights its relevance in the innate immune response.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human SECTM1 Protein (SEC-HM201) Specifications/Details

Protein	SECTM1
Synonyms	K-12; SECTM1
Accession	AAH17716
Species	Human
Biotinylated	no
Amino Acid Range	Gln29-Gly145
Molecular Weight	The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 47-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 4.46 uM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human SECTM1, hFc Tag captured on CM5 Chip via Protein A can bind Mouse CD7, His Tag with an affinity constant of 4.46 uM as determined in SPR assay (Biacore T200).
	Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
	Human SECTM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human SECTM1 is greater than 95% as determined by SEC-HPLC.

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