Product Description |
Recombinant Human SEMA3A/Semaphorin-3A Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asn21-Val771. |
Product Background |
The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain. |
Product Category |
Recombinant Protein |
Protein |
SEMA3A/Semaphorin-3A |
Synonyms |
Sema III; Semaphorin; Sema D; coll-1; collapsin 1; Hsema-I; Hsema-III; SEMA1, Sema3A; SEMAD; SEMAIII; SEMAL;Semaphorin 3A |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asn21-Val771 |
Molecular Weight |
The protein has a predicted MW of 87.7 kDa. Due to furin cleavage site, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-SEMA3A Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 1.30ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Anti-SEMA3A Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 1.30ug/ml determined by ELISA. |
|
|
Human SEMA3A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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