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Human Semaphorin 4A/SEMA4A Protein (SEM-HM14A) Summary
Product Description |
Recombinant Human Semaphorin 4A/SEMA4A Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly33-His683. |
Product Background |
Neuroimmune semaphorin 4A (Sema4A), a member of semaphorin family of transmembrane and secreted proteins, is an important regulator of neuronal and immune functions. In the nervous system, Sema4A primarily regulates the functional activity of neurons serving as an axon guidance molecule. In the immune system, Sema4A regulates immune cell activation and function, instructing a fine tuning of the immune response. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human Semaphorin 4A/SEMA4A Protein (SEM-HM14A) Specifications/Details
Protein |
Semaphorin 4A/SEMA4A |
Synonyms |
Semaphorin-4A; Semaphorin-B; SEMA4A |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly33-His683 |
Molecular Weight |
The protein has a predicted MW of 73.5 kDa. Due to glycosylation, the protein migrates to 85-95 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Semaphorin 4A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Semaphorin 4A is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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