Human Semaphorin 4A/SEMA4A PSI Domain Protein (SEM-HM2D2)

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Asn496-Ala548

Catalog #SEM-HM2D2

Price

$420⁰⁰

Size

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Human Semaphorin 4A/SEMA4A PSI Domain Protein (SEM-HM2D2) Summary

Product Description

Recombinant Human Semaphorin 4A/SEMA4A PSI Domain Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Asn496-Ala548.

Product Background

Neuroimmune semaphorin 4A (Sema4A), a member of semaphorin family of transmembrane and secreted proteins, is an important regulator of neuronal and immune functions. In the nervous system, Sema4A primarily regulates the functional activity of neurons serving as an axon guidance molecule. In the immune system, Sema4A regulates immune cell activation and function, instructing a fine tuning of the immune response.

Human Semaphorin 4A/SEMA4A PSI Domain Protein (SEM-HM2D2) Specifications/Details

Protein	Semaphorin 4A/SEMA4A
Synonyms	Semaphorin-4A; Semaphorin-B; SEMA4A
Accession	Q9H3S1-1
Species	Human
Biotinylated	no
Amino Acid Range	Asn496-Ala548
Molecular Weight	The protein has a predicted MW of 31.92 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human Semaphorin 4A PSI Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Semaphorin 4A PSI Domain is greater than 95% as determined by SEC-HPLC.