Human Semaphorin 4C/SEMA4C Protein (SEM-HM14C) Summary
Product Description |
Recombinant Human Semaphorin 4C/SEMA4C Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala21-Gly663. |
Product Background |
Semaphorin 4C (Sema4C), a type IV semaphorin, interacts with its high-affinity receptor PlexinB2 and has been recently demonstrated to participate in organ development, immune regulation, tumor growth and metastasis, pathological pain, and neural plasticity. |
Human Semaphorin 4C/SEMA4C Protein (SEM-HM14C) Specifications/Details
Protein |
Semaphorin 4C/SEMA4C |
Synonyms |
Semaphorin-4C; KIAA1739, SEMAI; SEMA4C; Semacl; Semaf; M-Sema F; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala21-Gly663 |
Molecular Weight |
The protein has a predicted MW of 73.58 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Other Available Proteins: