• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
  • Amino Acid Range: Met22-Arg734
Catalog #SEM-HM14D
Price
Regular price $33000
Regular price Sale price $33000
/
Size
Avantor VWR Fisher Scientific

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Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM14D) Summary

Product Description

 Recombinant Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Met22-Arg734.

Product Background

 Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.

Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM14D) Specifications/Details

Protein
 Semaphorin 4D/SEMA4D/CD100
Synonyms
 Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met22-Arg734
Molecular Weight
 The protein has a predicted MW of 80.77 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
Human Semaphorin 4D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Semaphorin 4D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC. The purity of Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...