Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44D)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Semaphorin 4D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met22-Arg734

Catalog: SEM-HM44D

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Product Description Recombinant Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Met22-Arg734.
Product Category Recombinant Protein
Target Semaphorin 4D/SEMA4D/CD100
Synonyms Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4
Gene/Protein ID Q92854-1
Species Human
Exact Sequence Met22-Arg734
Molecular Weight The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Semaphorin 4D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Semaphorin 4D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
The purity of Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.
Human Semaphorin 4D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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