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Non-biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44D) Summary
Product Description |
Recombinant Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Arg734. |
Product Background |
Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44D) Specifications/Details
Protein |
Semaphorin 4D/SEMA4D/CD100 |
Synonyms |
Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met22-Arg734 |
Molecular Weight |
The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. |
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Human Semaphorin 4D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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