Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44D)
Catalog: SEM-HM44D
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| Product Description | Recombinant Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Met22-Arg734. |
| Product Category | Recombinant Protein |
| Target | Semaphorin 4D/SEMA4D/CD100 |
| Synonyms | Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4 |
| Gene/Protein ID | Q92854-1 |
| Species | Human |
| Exact Sequence | Met22-Arg734 |
| Molecular Weight | The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Semaphorin 4D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Semaphorin 4D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. |
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The purity of Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC. |
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Human Semaphorin 4D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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