Human SERPINF2/A2AP Protein (SPF-HM101)
Human SERPINF2/A2AP Protein (SPF-HM101)
Human SERPINF2/A2AP Protein (SPF-HM101)
Human SERPINF2/A2AP Protein (SPF-HM101)
Human SERPINF2/A2AP Protein (SPF-HM101)
Human SERPINF2/A2AP Protein (SPF-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human SERPINF2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 45.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met28-Lys491
Catalog #SPF-HM101
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Product Description
 Recombinant Human SERPINF2/A2AP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met28-Lys491.
Product Background
 Large-pool solvent/detergent (SD) plasma for transfusion exhibits reduced alpha 2-antiplasmin (alpha2-AP; SERPINF2) functional activity. The reason for the loss of alpha2-AP has not been described and could be due to the SD incubation itself and/or to the processing steps implemented to remove the solvent and the detergent.
Product Category
 Recombinant Protein
Target
 SERPINF2/A2AP
Synonyms
 Alpha-2-AP; AAP; PLI; SERPINF2; A2AP; ALPHA-2-PI; API; Serpin F2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met28-Lys491
Molecular Weight
 The protein has a predicted MW of 52.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human SERPINF2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 45.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human SERPINF2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 45.9ng/ml determined by ELISA.
Human SERPINF2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SERPINF2 is greater than 90% as determined by SEC-HPLC.
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