Product Description |
Recombinant Human Serum Albumin (V418M, T420A, E505G, V547A) Protein is expressed from HEK293 without tag.It contains Asp25-Leu609 (V418M, T420A, E505G, V547A). |
Product Background |
Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier. |
Product Category |
Recombinant Protein |
Target |
Serum Albumin (V418M, T420A, E505G, V547A) |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp25-Leu609 (V418M, T420A, E505G, V547A) |
Molecular Weight |
The protein has a predicted MW of 66.37 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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