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Human Serum Albumin (V418M, T420A, E505G, V547A) Protein (BSA-HM0M1) Summary
Product Description |
Recombinant Human Serum Albumin (V418M, T420A, E505G, V547A) Protein is expressed from HEK293 without tag.It contains Asp25-Leu609 (V418M, T420A, E505G, V547A). |
Product Background |
Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Serum Albumin (V418M, T420A, E505G, V547A) Protein (BSA-HM0M1) Specifications/Details
Protein |
Serum Albumin |
Synonyms |
Albumin; ALB; Serum albumin; ANALBA; FDAH; PRO0883; PRO0903; PRO1341 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp25-Leu609 (V418M, T420A, E505G, V547A) |
Molecular Weight |
The protein has a predicted MW of 66.37 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
No Tag |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Serum Albumin (V418M, T420A, E505G, V547A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Serum Albumin (V418M, T420A, E505G, V547A) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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