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Human SEZ6 Sushi4&5 Domain Protein (SEZ-HM12D) Summary
Product Description |
Recombinant Human SEZ6 Sushi4&5 Domain Protein is expressed from HEK293 with His tag at the C-terminus.It contains Thr769-Ser899. |
Product Background |
Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human SEZ6 Sushi4&5 Domain Protein (SEZ-HM12D) Specifications/Details
Protein |
SEZ6 |
Synonyms |
SEZ-6; SEZ6; BSRPC; BSRP-C |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr769-Ser899 |
Molecular Weight |
The protein has a predicted MW of 15.1 kDa. Due to glycosylation, the protein migrates to 17-23 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SEZ6 Sushi4&5 Domain, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-SEZ6 Sushi4 Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human SEZ6 Sushi4&5 Domain, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-SEZ6 Sushi4 Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. |
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Human SEZ6 Sushi4&5 Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human SEZ6 Sushi4&5 Domain is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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