Western blot showing the expression of a protein with various molecular weights. The bands, represented at different levels, indicate the presence of the target protein at the expected molecular weight, with the marker bands for size comparison.
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Amino Acid Range: Ser32-Lys314
Catalog #SFP-HM101
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Size
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Human SFRP1/SARP2 Protein (SFP-HM101) Summary

Product Description

 Recombinant Human SFRP1/SARP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser32-Lys314.

Product Background

 Secreted frizzled-related protein 1 (SFRP1) is a gene that belongs to the secreted glycoprotein SFRP family. SFRP1 has been classified as a tumor suppressor gene due to the loss of expression in various human cancers, which is mainly attributed by epigenetic inactivation via DNA methylation or transcriptional silencing by microRNAs.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human SFRP1/SARP2 Protein (SFP-HM101) Specifications/Details

Protein
 SFRP1/SARP2
Synonyms
 Secreted frizzled-related protein 1; FRP-1; FRP1; sFRP-1; Secreted apoptosis-related protein 2; SARP-2; FRP; FrzA; sFRP; SARP
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser32-Lys314
Molecular Weight
 The protein has a predicted MW of 33.63 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human SFRP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human SFRP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SFRP1 is greater than 90% as determined by SEC-HPLC. The purity of Human SFRP1 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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support@kactusbio.us

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