HPLC chromatogram showing a major peak at retention time 10.3459 minutes, indicating compound elution and relative purity assessment.
Human SFRP2 Protein (SRP-HM102)
HPLC chromatogram showing a major peak at retention time 10.3459 minutes, indicating compound elution and relative purity assessment.
Human SFRP2 Protein (SRP-HM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Amino Acid Range: Leu25-Cys295
Catalog #SRP-HM102
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Size
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Human SFRP2 Protein (SRP-HM102) Summary

Product Description

 Recombinant Human SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Cys295.

Product Background

 As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human SFRP2 Protein (SRP-HM102) Specifications/Details

Protein
 SFRP2
Synonyms
 sFRP-2; SARP-1; Sarp1; Sdf5; Sfrp2; AI851596
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu25-Cys295
Molecular Weight
 The protein has a predicted MW of 32.4 kDa. Due to glycosylation, the protein migrates to 34-38 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human SFRP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human SFRP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SFRP2 is greater than 90% as determined by SEC-HPLC. The purity of Human SFRP2 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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